Nrf2 | HUABIO HA751401 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Nrf2

catalog :

HA751401

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH11-20

reactivity :

human, mouse, rat

application :

western blot, chromatin immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (HA751401) at 1/2,000 dilution. Lane 1: MEF cell lysate Lane 2: MEF treated with 10μM MG-132 for 8 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 100 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751401) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (HA751401) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 2μM MG-132 for 18 hours cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 25μM MG-132 for 4 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751401) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of MEF cells untreated / treated with 2μM MG-132 for 18 hours labeling Nrf2 with Rabbit anti-Nrf2 antibody (HA751401) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nrf2 antibody (HA751401) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA751401

Target name :

Nrf2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Nrf2 aa 51-350.

Uniprot id :

Q16236>SwissProt: Q16236 Human;SwissProt: Q60795 Mouse;SwissProt: O54968 Rat

Host :

Rabbit

Clone number :

PSH11-20

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MEF treated with 10μM MG-132 for 8 hours cell lysate, HeLa treated with 2μM MG-132 for 18 hours cell lysate, C6 treated with 25μM MG-132 for 4 hours cell lysate, MEF cells treated with 2μM MG-132 for 18 hours, HCT 116 cells treated with 25μM MG-132 for 4 hours.

Molecular wt :

Predicted band size: 68 kDa

Subcellular location :

Cytoplasm, cytosol, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751401_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HCT 116 cells untreated / treated with 25μM MG-132 for 4 hours labeling Nrf2 with Rabbit anti-Nrf2 antibody (HA751401) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nrf2 antibody (HA751401) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751401_5.jpg

Pic legend5 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from MEF cells treated with 10μM MG-132 for 8 hours with Nrf2 (HA751401) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage