product summary
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company name :
HUABIO
product type :
antibody
product name :
Presenilin 1 / PS-1
catalog :
HA751400
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH11-19
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (HA751400) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: MEF cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: HL-60 cell lysate (20 µg/Lane)
Lane 6: Daudi cell lysate (20 µg/Lane)
Lane 7: Neuro-2a cell lysate (20 µg/Lane)
Lane 8: Mouse brain tissue lysate (40 µg/Lane)
Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 10: Rat brain tissue lysate (40 µg/Lane)
Lane 11: Rat cerebellum tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 18 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751400) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751400 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751400 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA751400 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA751400 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801
product information
SKU :
HA751400
Target name :
Presenilin 1 / PS-1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human Presenilin 1 aa 311-360.
Uniprot id :
P49768>SwissProt: P49768 Human;SwissProt: P49769 Mouse;SwissProt: P97887 Rat
Host :
Rabbit
Clone number :
PSH11-19
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, HEK-293 cell lysate, MEF cell lysate, HepG2 cell lysate, HL-60 cell lysate, Daudi cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cytoplasmic granule, Cell membrane, Cell projection, growth cone, Early endosome, Early endosome membrane, neuron projection, axon, Synapse.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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