IL-8 | HUABIO HA751386 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

IL-8

catalog :

HA751386

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-96

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (HA751386) at 1/2,000 dilution. Lane 1: U-937 cell lysate Lane 2: U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751386) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of U-937 cells untreated (left) / treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours (right) labeling IL-8. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751386, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA751386

Target name :

IL-8

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IL-8 aa 1-99.

Uniprot id :

P10145>SwissProt: P10145 Human

Host :

Rabbit

Clone number :

PSH10-96

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate, U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours.

Molecular wt :

Predicted band size: 11 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage