product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
Cx40 / GJA5
catalog :
HA751374
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH10-82
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751374 image 1
Western blot analysis of Cx40 / GJA5 on different lysates with Rabbit anti-Cx40 / GJA5 antibody (HA751374) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: F9 cell lysate (20 µg/Lane) Lane 5: COS-1 cell lysate (20 µg/Lane) Lane 6: Mouse lung tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751374) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751374 image 2
Cx40 / GJA5 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA751374 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751374 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA751374 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA751374 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801
product information
SKU :
HA751374
Target name :
Cx40 / GJA5
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human GJA5 aa 227-358.
Uniprot id :
P36382>SwissProt: P36382 Human;SwissProt: Q01231 Mouse;SwissProt: P28234 Rat
Host :
Rabbit
Clone number :
PSH10-82
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HEK-293 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, F9 cell lysate, COS-1 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate.
Molecular wt :
Predicted band size: 40 kDa
Subcellular location :
Cell membrane, Cell junction, gap junction.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000 ;IP: 1-2μg/sample
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!