SV2A | HUABIO HA751371 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SV2A

catalog :

HA751371

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-69

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SV2A antibody (HA751371) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751371) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751371

Target name :

SV2A

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IP

Conjugate :

Non-conjugated

Uniprot id :

Q7L0J3>SwissProt: Q7L0J3 Human;SwissProt: Q9JIS5 Mouse;SwissProt: Q02563 Rat

Host :

Rabbit

Clone number :

PSH10-69

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human cerebellum tissue, mouse cerebellum tissue, mouse pancreas tissue, rat cerebellum tissue, rat pancreas tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 83 kDa

Subcellular location :

Presynapse, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:500-1:2,000 ;IHC-Fr: 1:500 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751371_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-SV2A antibody (HA751371) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751371_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-SV2A antibody (HA751371) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751371) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751371_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SV2A antibody (HA751371) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751371) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751371_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-SV2A antibody (HA751371) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751371_8.jpg

Pic legend8 :

Western blot analysis of SV2A on different lysates with Rabbit anti-SV2A antibody (HA751371) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: NIH/3T3 cell lysate (negative) Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751371) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751371_9.jpg

Pic legend9 :

SV2A was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751371 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751371 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brain tissue lysate (input) Lane 2: HA751371 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA751371 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801

more info or order :

HUABIO product webpage