product summary
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company name :
HUABIO
product type :
antibody
product name :
BLBP
catalog :
HA751370
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH10-68
reactivity :
mouse, rat
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
image
image 1 :
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required
image 2 :
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex (P7)
Sample: Paraffin-embedded section
Antibody concentration: 1:1,000
image 3 :
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1:1,000
product information
SKU :
HA751370
Target name :
BLBP
Species reactivity :
Mouse,Rat,Cynomolgus monkey,Pig
Applications :
WB,IHC-P,IHC-Fr,IF-Tissue
Conjugate :
Non-conjugated
Uniprot id :
P51880>SwissProt: P51880 Mouse;SwissProt: P55051 Rat
Host :
Rabbit
Clone number :
PSH10-68
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse P7 brain tissue, mouse cerebellum tissue, Mouse cerebellum (P0) tissue lysate, Mouse cerebellum (P7) tissue lysate, Rat cerebellum tissue lysate.
Molecular wt :
Predicted band size: 15 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000
;IHC-P: 1:2,000
;IHC-Fr: 1:500
;IF-Tissue: 1:1,000
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751370_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse P7 brain tissue with Rabbit anti-BLBP antibody (HA751370) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751370) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751370_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse P7 brain tissue with Rabbit anti-BLBP antibody (HA751370) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751370) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA751370_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-BLBP antibody (HA751370) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751370) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA751370_7.jpg
Pic legend7 :
Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA751370) at 1/5,000 dilution.
Lane 1: Mouse cerebellum (P0) tissue lysate
Lane 2: Mouse cerebellum (P7) tissue lysate
Lane 3: Mouse kidney tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751370) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA751370_8.jpg
Pic legend8 :
Western blot analysis of BLBP on different lysates with Rabbit anti-BLBP antibody (HA751370) at 1/5,000 dilution.
Lane 1: Rat cerebellum tissue lysate
Lane 2: Rat kidney tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751370) at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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