product summary
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company name :
HUABIO
product type :
antibody
product name :
VPS34
catalog :
HA751367
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH10-64
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (HA751367) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: PC-3 cell lysate (20 µg/Lane)
Lane 3: C2C12 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 98 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751367) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of VPS34 on different lysates with Rabbit anti-VPS34 antibody (HA751367) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-VPS34 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 98 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751367) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA751367
Target name :
VPS34
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human VPS34 aa 251-887.
Uniprot id :
Q8NEB9>SwissProt: Q8NEB9 Human;SwissProt: Q6PF93 Mouse;SwissProt: O88763 Rat
Host :
Rabbit
Clone number :
PSH10-64
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, PC-3 cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Mouse brain tissue lysate, Rat heart tissue lysate.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Midbody, Late endosome, Cytoplasmic vesicle, autophagosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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