NOX2 / gp91phox | HUABIO HA751362 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NOX2 / gp91phox

catalog :

HA751362

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-59

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse liver tissue with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751362, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 2 :

Immunofluorescence analysis of frozen rat liver tissue with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751362, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751362

Target name :

NOX2 / gp91phox

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,IHC-Fr,IP

Conjugate :

Non-conjugated

Uniprot id :

P04839>SwissProt: P04839 Human;SwissProt: Q61093 Mouse;Entrez Gene: 66021 Rat

Host :

Rabbit

Clone number :

PSH10-59

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

RAW264.7 cell lysate, Mouse spleen tissue lysate, RAW264.7, human liver tissue, mouse liver tissue, rat liver tissue.

Molecular wt :

Predicted band size: 65 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:500 ;IHC-P: 1:1,000 ;IHC-Fr: 1:500 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751362_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751362) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751362_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751362_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (negative) with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751362_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751362_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue (negative) with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751362_9.jpg

Pic legend9 :

Western blot analysis of NOX2 / gp91phox on different lysates with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (negative) (20 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Predicted band size: 65 kDa Observed band size: 55 kDa Exposure time: Lane 1: 16 seconds; Lane 2-3: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751362) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA751362_10.jpg

Pic legend10 :

Immunocytochemistry analysis of RAW264.7 (positive) and Neuro-2a (negative) labeling NOX2 / gp91phox with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NOX2 / gp91phox antibody (HA751362) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA751362_11.jpg

Pic legend11 :

NOX2 / gp91phox was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA751362 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751362 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: HA751362 IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of HA751362 in RAW264.7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 3 seconds; ECL: K1801

more info or order :

HUABIO product webpage