TCF7 | HUABIO HA751340 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TCF7

catalog :

HA751340

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-10

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TCF7 on different lysates with Rabbit anti-TCF7 antibody (HA751340) at 1/2,000 dilution. Lane 1: MOLT-4 cell lysate Lane 2: COLO205 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 45-50 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751340) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-TCF7 antibody (HA751340) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751340) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsils tissue with Rabbit anti-TCF7 antibody (HA751340) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751340) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751340

Target name :

TCF7

Species reactivity :

Human

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TCF7 aa 1-300.

Uniprot id :

P36402>SwissProt: P36402 Human

Host :

Rabbit

Clone number :

PSH10-10

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MOLT-4 cell lysate, COLO205 cell lysate, Jurkat cell lysate, human thymus tissue, human tonsils tissue.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751340_4.jpg

Pic legend4 :

TCF7 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA751340 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751340 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA751340 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA751340 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 seconds; ECL: K1801

more info or order :

HUABIO product webpage