product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-EGFR (T669)
catalog :
HA751333
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH10-03
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751333 image 1
Western blot analysis of Phospho-EGFR (T669) on different lysates with Rabbit anti-Phospho-EGFR (T669) antibody (HA751333) at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 134 kDa Observed band size: 150 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751333) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751333 image 2
Phospho-EGFR (T669) was immunoprecipitated from 0.2 mg A431 starved overnight then treated with 100ng/mL EGF for 15 minutes cell lysate with HA751333 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751333 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 starved overnight then treated with 100ng/mL EGF for 15 minutes cell lysate (input) Lane 2: HA751333 IP in A431 starved overnight then treated with 100ng/mL EGF for 15 minutes cell lysate Lane 3: Rabbit IgG instead of HA751333 in A431 starved overnight then treated with 100ng/mL EGF for 15 minutes cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801
product information
SKU :
HA751333
Target name :
Phospho-EGFR (T669)
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Thr669 of Human EGFR.
Uniprot id :
P00533>SwissProt: P00533 Human
Host :
Rabbit
Clone number :
PSH10-03
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A431 treated with 100ng/mL EGF for 30 minutes cell lysate.
Molecular wt :
Predicted band size: 134 kDa
Subcellular location :
Cell membrane, Nucleus membrane, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane, Endosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000 ;IP: 1-2μg/sample
Advanced Validation :
Cell treatment (CT)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!