CD40 | HUABIO HA751331 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD40

catalog :

HA751331

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-01

reactivity :

human, mouse, rat

application :

flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Spleen Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 3 :

Application: IF-Tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500

product information

SKU :

HA751331

Target name :

CD40

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

IHC-P,IHC-Fr,IF-Cell,FC,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD40 aa 228-277 / 277.

Uniprot id :

P25942>SwissProt: P25942 Human;SwissProt: P27512 Mouse;Entrez Gene: 171369 Rat

Host :

Rabbit

Clone number :

PSH10-01

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human spleen tissue, mouse spleen tissue, rat spleen tissue, Raji, A20, PC-12.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cell membrane. Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P:1:1,000 ;IHC-Fr:1:1,000 ;IF-Cell:1:50-1:500 ;FC:1:1,000 ;IF-Tissue: 1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751331_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD40 antibody (HA751331) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751331) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751331_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD40 antibody (HA751331) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751331) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751331_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD40 antibody (HA751331) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751331) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751331_7.jpg

Pic legend7 :

Immunocytochemistry analysis of Raji cells labeling CD40 with Rabbit anti-CD40 antibody (HA751331) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD40 antibody (HA751331) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751331_8.jpg

Pic legend8 :

Immunocytochemistry analysis of A20 cells labeling CD40 with Rabbit anti-CD40 antibody (HA751331) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD40 antibody (HA751331) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751331_9.jpg

Pic legend9 :

Immunocytochemistry analysis of PC-12 cells labeling CD40 with Rabbit anti-CD40 antibody (HA751331) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD40 antibody (HA751331) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA751331_10.jpg

Pic legend10 :

Flow cytometric analysis of A20 cells labeling CD40. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751331, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage