RecA | HUABIO HA751327 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RecA

catalog :

HA751327

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH09-96

reactivity :

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RecA on E.coli lysates with Rabbit anti-RecA antibody (HA751327) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 38 kDa Observed band size: 42 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751327) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of E.coli labeling RecA with Rabbit anti-RecA antibody (HA751327) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RecA antibody (HA751327) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA751327

Target name :

RecA

Species reactivity :

Escherichia coli

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within becterium RecA aa 1-353.

Uniprot id :

P0A7G6>SwissProt: P0A7G6 Escherichia coli

Host :

Rabbit

Clone number :

PSH09-96

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

E.coli lysates, E.coli.

Molecular wt :

Predicted band size: 38 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100

more info or order :

HUABIO product webpage