Dopamine D2 Receptor | HUABIO HA751325 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Dopamine D2 Receptor

catalog :

HA751325

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH09-94

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse striatum tissue with Rabbit anti-Dopamine D2 Receptor antibody (HA751325) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751325, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 2 :

Immunohistochemical analysis of paraffin-embedded human cerebral cortex (blood vessel) tissue with Rabbit anti-Dopamine D2 Receptor antibody (HA751325) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751325) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-Dopamine D2 Receptor antibody (HA751325) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751325) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751325

Target name :

Dopamine D2 Receptor

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-Fr,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Dopamine D2 Receptor aa 214-373.

Uniprot id :

P14416>SwissProt: P14416 Human;SwissProt: P61168 Mouse;SwissProt: P61169 Rat

Host :

Rabbit

Clone number :

PSH09-94

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human cerebral cortex (blood vessel) tissue, mouse brain (blood vessel) tissue, mouse striatum tissue, rat brain (blood vessel) tissue, rat striatum tissue.

Molecular wt :

Predicted band size: 51 kDa

Subcellular location :

Cell membrane, Golgi apparatus membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:200

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751325_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain (blood vessel) tissue with Rabbit anti-Dopamine D2 Receptor antibody (HA751325) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751325) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751325_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Dopamine D2 Receptor antibody (HA751325) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751325) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751325_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain (blood vessel) tissue with Rabbit anti-Dopamine D2 Receptor antibody (HA751325) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751325) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751325_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rabbit anti-Dopamine D2 Receptor antibody (HA751325) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751325) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage