product summary
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company name :
HUABIO
product type :
antibody
product name :
Dopamine D2 Receptor
catalog :
HA751324
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH09-93
reactivity :
human, mouse
application :
western blot, flow cytometry, immunohistochemistry - frozen section
more info or order :
image
image 1 :
Western blot analysis of Dopamine D2 Receptor on different lysates with Rabbit anti-Dopamine D2 Receptor antibody (HA751324) at 1/10,000 dilution.
Lane 1: SH-SY5Y cell lysate (no heat)
Lane 2: U-87 MG cell lysate (no heat)
Lane 3: NCI-H1299 cell lysate (no heat)
Lane 4: THP-1 cell lysate (no heat)
Lane 5: Neuro-2a cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751324) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunofluorescence analysis of frozen mouse striatum tissue with Rabbit anti-Dopamine D2 Receptor antibody (HA751324) at 1/500 dilution.
The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751324, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
image 3 :
Immunocytochemistry analysis of SH-SY5Y cells labeling Dopamine D2 Receptor with Rabbit anti-Dopamine D2 Receptor antibody (HA751324) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dopamine D2 Receptor antibody (HA751324) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA751324
Target name :
Dopamine D2 Receptor
Species reactivity :
Human,Mouse
Applications :
WB,IHC-Fr,IF-Cell,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human Dopamine D2 Receptor aa 214-373.
Uniprot id :
P14416>SwissProt: P14416 Human;SwissProt: P61168 Mouse
Host :
Rabbit
Clone number :
PSH09-93
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y cell lysate, U-87 MG cell lysate, NCI-H1299 cell lysate, THP-1 cell lysate, Neuro-2a cell lysate, mouse striatum tissue, SH-SY5Y, Neuro-2a.
Molecular wt :
Predicted band size: 51 kDa
Subcellular location :
Cell membrane, Golgi apparatus membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:10,000
;IHC-Fr: 1:500
;IF-Cell: 1:100
;FC: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751324_4.jpg
Pic legend4 :
Immunocytochemistry analysis of Neuro-2a cells labeling Dopamine D2 Receptor with Rabbit anti-Dopamine D2 Receptor antibody (HA751324) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dopamine D2 Receptor antibody (HA751324) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751324_5.jpg
Pic legend5 :
Flow cytometric analysis of SH-SY5Y cells labeling Dopamine D2 Receptor.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751324, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA751324_6.jpg
Pic legend6 :
Flow cytometric analysis of Neuro-2a cells labeling Dopamine D2 Receptor.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751324, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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