Phospho-STING (S366) | HUABIO HA751314 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-STING (S366)

catalog :

HA751314

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH09-72

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, dot blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-STING (S366) on different lysates with Rabbit anti-Phospho-STING (S366) antibody (HA751314) at 1/2,000 dilution. Lane 1: THP-1 treated with 80nM TPA for 16 hours cell lysate Lane 2: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate Lane 3: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751314) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Dot blot analysis of Phospho-STING (S366) on different peptides with Rabbit anti-Phospho-STING (S366) antibody (HA751314) at 1/2,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified STING peptide (negative) Lane 2: Phospho-STING (S366) peptide (positive) Proteins loading: 100ng, 50ng, 10ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute; ECL: K1801.

image 3 :

Phospho-STING (S366) was immunoprecipitated from 0.2 mg THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate with HA751314 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751314 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate (input) Lane 2: HA751314 IP in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate Lane 3: Rabbit IgG instead of HA751314 in THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801

product information

SKU :

HA751314

Target name :

Phospho-STING (S366)

Species reactivity :

Human

Applications :

WB,Dot Blot,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser366 of Human STING.

Uniprot id :

Q86WV6>SwissProt: Q86WV6 Human;SwissProt: Q3TBT3 Mouse;SwissProt: F1M391 Rat

Host :

Rabbit

Clone number :

PSH09-72

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 treated with 80nM TPA for 16 hours then transfected with 5μg/mL Poly (dA:dT) for 3 hours cell lysate.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Endoplasmic reticulum membrane, Cytoplasm, perinuclear region, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Cytoplasmic vesicle, autophagosome membrane, Mitochondrion outer membrane, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;Dot Blot: 1:2,000 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage