eIF3g | HUABIO HA751288 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

eIF3g

catalog :

HA751288

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH09-41

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of eIF3g on different lysates with Rabbit anti-eIF3g antibody (HA751288) at 1/25,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 45 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751288) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling eIF3g with Rabbit anti-eIF3g antibody (HA751288) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF3g antibody (HA751288) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Flow cytometric analysis of HeLa cells labeling eIF3g. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751288, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA751288

Target name :

eIF3g

Species reactivity :

Human

Applications :

WB,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human eIF3g aa 1-320.

Uniprot id :

O75821>SwissProt: O75821 Human

Host :

Rabbit

Clone number :

PSH09-41

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K-562 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, HeLa.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Cytoplasm, Nucleus, perinuclear region.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:25,000 ;IF-Cell: 1:100 ;FC: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751288_4.jpg

Pic legend4 :

eIF3g was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with HA751288 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751288 at 1/5,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y cell lysate (input) Lane 2: HA751288 IP in SH-SY5Y cell lysate Lane 3: Rabbit IgG instead of HA751288 in SH-SY5Y cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 12 seconds; ECL: K1801

more info or order :

HUABIO product webpage