DMT1 | HUABIO HA751274 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DMT1

catalog :

HA751274

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH09-20

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DMT1 on different lysates with Rabbit anti-DMT1 antibody (HA751274) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (no heat) Lane 2: HepG2 cell lysate (no heat) Lane 3: HeLa cell lysate (no heat) Lane 4: HeLa cell lysate (hot lysis) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 75 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751274) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DMT1 antibody (HA751274) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751274) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Flow cytometric analysis of SH-SH5Y cells labeling DMT1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751274, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA751274

Target name :

DMT1

Species reactivity :

Human

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human DMT1 aa 1-400.

Uniprot id :

P49281>SwissProt: P49281 Human

Host :

Rabbit

Clone number :

PSH09-20

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, HepG2 cell lysate, HeLa cell lysate, human brain tissue, SH-SH5Y.

Molecular wt :

Predicted band size: 62 kDa

Subcellular location :

Mitochondrion outer membrane, Golgi apparatus, trans-Golgi network membrane, Recycling endosome membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:1,000 ;FC: 1:1,000

more info or order :

HUABIO product webpage