product summary
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company name :
HUABIO
product type :
antibody
product name :
FOXG1
catalog :
HA751263
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH09-08
reactivity :
mouse
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :

Western blot analysis of FOXG1 on different lysates with Rabbit anti-FOXG1 antibody (HA751263) at 1/2,000 dilution.
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 58 kDa
Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751263) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :

FOXG1 was immunoprecipitated from 0.2 mg Neuro-2a cell lysate with HA751263 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751263 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Neuro-2a cell lysate (input)
Lane 2: HA751263 IP in Neuro-2a cell lysate
Lane 3: Rabbit IgG instead of HA751263 in Neuro-2a cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 seconds; ECL: K1801
product information
SKU :
HA751263
Target name :
FOXG1
Species reactivity :
Mouse
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse FOXG1 aa 1-200.
Uniprot id :
Q60987>SwissProt: Q60987 Mouse
Host :
Rabbit
Clone number :
PSH09-08
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Neuro-2a cell lysate, Mouse brain tissue lysate.
Molecular wt :
Predicted band size: 52 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IP: 1-2μg/sample
more info or order :
company information

HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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