Phospho-SQSTM1 / p62 (S349) | HUABIO HA751258 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-SQSTM1 / p62 (S349)

catalog :

HA751258

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-97

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-SQSTM1 / p62 (S349) on different lysates with Rabbit anti-Phospho-SQSTM1 / p62 (S349) antibody (HA751258) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 2μM MG-132 for 18 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 62 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751258) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with 2μM MG-132 for 18 hours labeling Phospho-SQSTM1 / p62 (S349) with Rabbit anti-Phospho-SQSTM1 / p62 (S349) antibody (HA751258) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-SQSTM1 / p62 (S349) antibody (HA751258) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells treated with 10μM MG-132 for 8 hours labeling Phospho-SQSTM1 / p62 (S349) with Rabbit anti-Phospho-SQSTM1 / p62 (S349) antibody (HA751258) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-SQSTM1 / p62 (S349) antibody (HA751258) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA751258

Target name :

Phospho-SQSTM1 / p62 (S349)

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser349 of Human SQSTM1/ p62.

Uniprot id :

Q13501>SwissProt: Q13501 Human;SwissProt: Q64337 Mouse

Host :

Rabbit

Clone number :

PSH08-97

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 2μM MG-132 for 18 hours cell lysate, NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate, HeLa cells treated with 2μM MG-132 for 18 hours, NIH/3T3cells treated with 10μM MG-132 for 8 hours , human cerebrum tissue.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:5,000 ;IHC-P: 1:30,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751258_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue with Rabbit anti-Phospho-SQSTM1 / p62 (S349) antibody (HA751258) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751258) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage