TREX1 | HUABIO HA751249 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TREX1

catalog :

HA751249

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-84

reactivity :

mouse

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TREX1 on different lysates with Rabbit anti-TREX1 antibody (HA751249) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: CTLL-2 cell lysate Lane 3: J774A.1 cell lysate Lane 4: Neuro-2a cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: Lane 1: 25 seconds; Lane 2-4: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751249) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of Neuro-2a (left, negative) and RAW264.7 (right, positive) cells labeling TREX1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751249, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

TREX1 was immunoprecipitated from 0.2 mg RAW264.7 cell lysate with HA751249 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751249 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10,000 dilution was used for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate (input) Lane 2: HA751249 IP in RAW264.7 cell lysate Lane 3: Rabbit IgG instead of HA751249 in RAW264.7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 16 seconds; ECL: K1801

product information

SKU :

HA751249

Target name :

TREX1

Species reactivity :

Mouse

Applications :

WB,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse TREX1 aa 1-280.

Uniprot id :

Q91XB0>SwissProt: Q91XB0 Mouse

Host :

Rabbit

Clone number :

PSH08-84

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

RAW264.7 cell lysate, CTLL-2 cell lysate, J774A.1 cell lysate, RAW264.7.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Nucleus, Cytoplasm, cytosol, Endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage