Alpha-Synuclein | HUABIO HA751248 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Alpha-Synuclein

catalog :

HA751248

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-83

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

image 3 :

Western blot analysis of Alpha-Synuclein on different lysates with Rabbit anti-Alpha-Synuclein antibody (HA751248) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with Alpha-Synuclein cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 14 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751248) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA751248

Target name :

Alpha-Synuclein

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Alpha-Synuclein aa 1-140.

Uniprot id :

P37840>SwissProt: P37840 Human;SwissProt: O55042 Mouse;SwissProt: P37377 Rat

Host :

Rabbit

Clone number :

PSH08-83

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse cerebellum tissue, rat cerebellum tissue.

Molecular wt :

Predicted band size: 14 kDa

Subcellular location :

Cytoplasm, Membrane, Nucleus, Synapse, Secreted, Cell projection, axon.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IHC-P: 1:5,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:500-1:1,000

Advanced Validation :

Cell treatment (CT),Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751248_4.jpg

Pic legend4 :

Western blot analysis of Alpha-Synuclein on different lysates with Rabbit anti-Alpha-Synuclein antibody (HA751248) at 1/5,000 dilution. Lane 1: Human SCNA recombinant protein, 30ng/Lane Lane 2: Human SCNB recombinant protein, 30ng/Lane Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751248) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751248_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Alpha-Synuclein antibody (HA751248) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751248) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751248_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (low expression) with Rabbit anti-Alpha-Synuclein antibody (HA751248) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751248) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage