SRPK1 | HUABIO HA751217 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SRPK1

catalog :

HA751217

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-26

reactivity :

human, mouse

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SRPK1 on different lysates with Rabbit anti-SRPK1 antibody (HA751217) at 1/5,000 dilution. Lane 1: NCI-H226 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Caco-2 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: MDA-MB-231 cell lysate (20 µg/Lane) Lane 6: BxPC-3 cell lysate (20 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Predicted band size: 74 kDa Observed band size: 100 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751217) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Jurkat cells labeling SRPK1 with Rabbit anti-SRPK1 antibody (HA751217) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SRPK1 antibody (HA751217) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-SRPK1 antibody (HA751217) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751217) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751217

Target name :

SRPK1

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P,IF-Tissue,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human SRPK1 aa 251-500.

Uniprot id :

Q96SB4>SwissProt: Q96SB4 Human;SwissProt: O70551 Mouse;Entrez Gene: 361811 Rat

Host :

Rabbit

Clone number :

PSH08-26

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NCI-H226 cell lysate, Jurkat cell lysate, Caco-2 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, BxPC-3 cell lysate, Mouse testis tissue lysate, Jurkat, human breast tissue, human colon cancer tissue, human testis tissue, mouse testis tissue, NIH/3T3.

Molecular wt :

Predicted band size: 74 kDa

Subcellular location :

Cytoplasm, Nucleus, nucleoplasm, Nucleus speckle, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:500 ;IHC-P: 1:200-1:1,000 ;IF-Tissue: 1:200 ;FC: 1:1,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751217_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SRPK1 antibody (HA751217) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751217_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SRPK1 antibody (HA751217) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751217_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SRPK1 antibody (HA751217) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751217_7.jpg

Pic legend7 :

Flow cytometric analysis of Jurkat cells labeling SRPK1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751217, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751217_8.jpg

Pic legend8 :

Flow cytometric analysis of NIH/3T3 cells labeling SRPK1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751217, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751217_9.jpg

Pic legend9 :

SRPK1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA751217 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751217 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA751217 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA751217 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 7 seconds; ECL: K1801

more info or order :

HUABIO product webpage