Oxytocin-neurophysin 1 / OXT | HUABIO HA751215 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Oxytocin-neurophysin 1 / OXT

catalog :

HA751215

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-24

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Paraventricular nucleus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Supraoptic nucleus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Application: IF-tissue Species: Mouse Site: Pituitary Sample: Paraffin-embedded section Antibody concentration: 1/2,000

product information

SKU :

HA751215

Target name :

Oxytocin-neurophysin 1 / OXT

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human OXT aa 1-125.

Uniprot id :

P01178>SwissProt: P01178 Human;SwissProt: P35454 Mouse;Entrez Gene: 25504 Rat

Host :

Rabbit

Clone number :

PSH08-24

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse paraventricular nucleus tissue, rat supraoptic nucleus tissue, mouse pituitary tissue, rat pituitary tissue, Mouse pituitary tissue lysate, Rat pituitary tissue lysate.

Molecular wt :

Predicted band size: 13 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:2,000-1:5,000 ;IHC-Fr: 1:500-1:1,000 ;IF-Tissue: 1:2,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751215_4.jpg

Pic legend4 :

Application: IF-tissue Species: Rat Site: Pituitary Sample: Paraffin-embedded section Antibody concentration: 1/2,000

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751215_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse paraventricular nucleus tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751215_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751215_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751215_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751215_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue (negative) with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751215) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA751215_10.jpg

Pic legend10 :

Western blot analysis of Oxytocin-neurophysin 1 / OXT on different lysates with Rabbit anti-Oxytocin-neurophysin 1 / OXT antibody (HA751215) at 1/1,000 dilution. Lane 1: Mouse pituitary tissue lysate Lane 2: Rat pituitary tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 13 kDa Observed band size: 14 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751215) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage