product summary
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company name :
HUABIO
product type :
antibody
product name :
Hexokinase II
catalog :
HA751199
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH07-97
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Hexokinase II on different lysates with Rabbit anti-Hexokinase II antibody (HA751199) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HCT 116 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: COS-1 cell lysate (20 µg/Lane)
Lane 6: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 7: Mouse testis tissue lysate (40 µg/Lane)
Lane 8: Rat skeletal muscle tissue lysate (40 µg/Lane)
Lane 9: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751199) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of NIH/3T3 cells labeling Hexokinase II with Rabbit anti-Hexokinase II antibody (HA751199) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Hexokinase II antibody (HA751199) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunocytochemistry analysis of PC-12 cells labeling Hexokinase II with Rabbit anti-Hexokinase II antibody (HA751199) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Hexokinase II antibody (HA751199) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA751199
Target name :
Hexokinase II
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IHC-P,IF-Cell,FC,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within
Uniprot id :
P52789>SwissProt: P52789 Human;SwissProt: O08528 Mouse;SwissProt: P27881 Rat
Host :
Rabbit
Clone number :
PSH07-97
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, COS-1 cell lysate, Mouse skeletal muscle tissue lysate, Mouse testis tissue lysate, Rat skeletal muscle tissue lysate, Rat testis tissue lysate, NIH/3T3, PC-12, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Mitochondrion outer membrane, Cytoplasm, cytosol.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:50
;IF-Cell: 1:50-1:100
;FC: 1:1,000
;IP: 1-2μg/sample
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751199_4.jpg
Pic legend4 :
Flow cytometric analysis of NIH/3T3 cells labeling Hexokinase II.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751199, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751199_5.jpg
Pic legend5 :
Flow cytometric analysis of PC-12 cells labeling Hexokinase II.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751199, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA751199_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Hexokinase II antibody (HA751199) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751199) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA751199_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Hexokinase II antibody (HA751199) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751199) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA751199_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Hexokinase II antibody (HA751199) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751199) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA751199_9.jpg
Pic legend9 :
Hexokinase II was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751199 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751199 at 1/2,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA751199 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA751199 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 6 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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