LYVE1 | HUABIO HA751179 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

LYVE1

catalog :

HA751179

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-67

reactivity :

mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of LYVE1 on different lysates with Rabbit anti-LYVE1 antibody (HA751179) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse brain tissue lysate (negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 35 kDa Observed band size: 60 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LYVE1 antibody (HA751179) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751179) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751179

Target name :

LYVE1

Species reactivity :

Mouse

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse LYVE1 aa 1-250.

Uniprot id :

Q8BHC0>SwissProt: Q8BHC0 Mouse

Host :

Rabbit

Clone number :

PSH07-67

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse lung tissue lysate, mouse colon tissue.

Molecular wt :

Predicted band size: 35 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage