DDIT3 | HUABIO HA751178 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DDIT3

catalog :

HA751178

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-65

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (HA751178) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 2μg/mL tunicamycin for 8 hours cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 2μg/mL thapsigargin for 8 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 2μg/mL tunicamycin for 8 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-6: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751178) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with 2μg/mL tunicamycin for 8 hours labeling DDIT3 with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of C2C12 cells treated with 2μg/mL thapsigargin for 8 hours labeling DDIT3 with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA751178

Target name :

DDIT3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human DDIT3 aa 1-169.

Uniprot id :

P35638>SwissProt: P35638 Human;SwissProt: P35639 Mouse;SwissProt: Q62857 Rat

Host :

Rabbit

Clone number :

PSH07-65

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 2μg/mL tunicamycin for 8 hours cell lysate, C2C12 treated with 2μg/mL thapsigargin for 8 hours cell lysate, C6 cell lysate, C6 treated with 2μg/mL tunicamycin for 8 hours cell lysate, HeLa cells treated with 2μg/mL tunicamycin for 8 hours, C2C12 cells treated with 2μg/mL thapsigargin for 8 hours, C6 cells treated with 2μg/mL tunicamycin for 8 hours.

Molecular wt :

Predicted band size: 19 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751178_4.jpg

Pic legend4 :

Immunocytochemistry analysis of C6 cells treated with 2μg/mL tunicamycin for 8 hours labeling DDIT3 with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751178_5.jpg

Pic legend5 :

Flow cytometric analysis of C2C12 cells treated with 2μg/mL thapsigargin for 8 hours labeling DDIT3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751178, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage