product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-Cytosolic Phospholipase A2 (S505)
catalog :
HA751176
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH07-56
reactivity :
human, mouse
application :
western blot, dot blot
more info or order :
image
image 1 :
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751176) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751176) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751176) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Dot blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different proteins with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751176) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature.
Lane 1: Unmodified Cytosolic Phospholipase A2 (negative)
Lane 2: Phospho-Cytosolic Phospholipase A2 (S505) (positive)
Proteins loading: 50ng, 25ng, 5ng, 1ng;
Blocking and dilution buffer: 5% NFDM/TBST;
Exposure time: 6 seconds; ECL: K1801.
product information
SKU :
HA751176
Target name :
Phospho-Cytosolic Phospholipase A2 (S505)
Species reactivity :
Human,Mouse
Applications :
WB,Dot Blot
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser505 of human cPLA2 aa 491-540.
Uniprot id :
P47712>SwissProt: P47712 Human;SwissProt: P47713 Mouse
Host :
Rabbit
Clone number :
PSH07-56
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, RAW264.7 cell lysates.
Molecular wt :
Predicted band size: 85 kDa
Subcellular location :
Cytoplasm, Golgi apparatus membrane, Nucleus envelope.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;Dot Blot: 1:1,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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