c-Fos | HUABIO HA751172 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

c-Fos

catalog :

HA751172

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-51

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Hypothalamus (restraint stress induced) Sample: Frozen section Antibody concentration: 1/4,000 Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

image 2 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/4,000 Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

image 3 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/4,000 Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

product information

SKU :

HA751172

Target name :

c-Fos

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-Fr,IF-Cell,IHC-P,ChIP,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Protein c-Fos aa 1-380.

Uniprot id :

P01100>SwissProt: P01100 Human;SwissProt: P01101 Mouse;SwissProt: P12841 Rat

Host :

Rabbit

Clone number :

PSH07-51

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue, mouse brain tissue, mouse hippocampus tissue, HeLa cells serum starved for 40 hours then add 20% FBS for 2 hours, RAW264.7 cells serum starved for 16 hours then add 20% FBS for 4 hours, C6 cells serum starved for 16 hours then add 10% FBS for 30 minutes, HeLa serum starved for 40 hours then add 20% FBS for 2 hours cell lysate, RAW264.7 serum starved for 16 hours then add 200nM PMA for 4 hours cell lysate, C6 serum starved for 16 hours then add 10% FBS for 30 minutes cell lysate.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IHC-Fr: 1:4,000-1:10,000 ;IF-Cell: 1:1,000-1:2,000 ;IHC-P: 1:5,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;IF-Tissue: 1:4000-1:10,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751172_4.jpg

Pic legend4 :

Western blot analysis of c-Fos on different lysates with Rabbit anti-c-Fos antibody (HA751172) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 41-55 kDa Exposure time: 10 seconds; ECL: K1801; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751172) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751172_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse brain (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA751172) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751172) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751172_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA751172) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751172) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751172_7.jpg

Pic legend7 :

Immunocytochemistry analysis of HeLa cells serum starved for 40 hours then add 20% FBS for 2 hours labeling c-Fos with Rabbit anti-c-Fos antibody (HA751172) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Fos antibody (HA751172) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751172_8.jpg

Pic legend8 :

Immunocytochemistry analysis of RAW264.7 cells serum starved for 16 hours then add 20% FBS for 4 hours labeling c-Fos with Rabbit anti-c-Fos antibody (HA751172) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Fos antibody (HA751172) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751172_9.jpg

Pic legend9 :

Immunocytochemistry analysis of C6 cells serum starved for 16 hours then add 10% FBS for 30 minutes labeling c-Fos with Rabbit anti-c-Fos antibody (HA751172) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Fos antibody (HA751172) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA751172_10.jpg

Pic legend10 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells (serum starved for 16 hours and treated with 200 nM TPA for 4 hours) with c-Fos (HA751172) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA751172_11.jpg

Pic legend11 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Paraffin-embedded section Antibody concentration: 1/4,000 Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA751172_12.jpg

Pic legend12 :

c-Fos was immunoprecipitated from 0.2 mg HeLa serum starved for 40 hours then add 20% FBS for 2 hours cell lysate with HA751172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751172 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa serum starved for 40 hours then add 20% FBS for 2 hours cell lysate (input) Lane 2: HA751172 IP in HeLa serum starved for 40 hours then add 20% FBS for 2 hours cell lysate Lane 3: Rabbit IgG instead of HA751172 in HeLa serum starved for 40 hours then add 20% FBS for 2 hours cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 59 seconds; ECL: K1801

more info or order :

HUABIO product webpage