Von Willebrand Factor | HUABIO HA751166 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Von Willebrand Factor

catalog :

HA751166

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-45

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (negative) Lane 2: Human lung tissue lysate Lane 3: Rat lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: Lane 1-3 (left): 25 seconds; Lane 1-3 (right): 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751166) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751166

Target name :

Von Willebrand Factor

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Von Willebrand Factor aa 734-1,283.

Uniprot id :

P04275>SwissProt: P04275 Human;SwissProt: Q8CIZ8 Mouse;SwissProt: A0A8J8XVZ5 Rat

Host :

Rabbit

Clone number :

PSH07-45

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human lung tissue lysate, Rat lung tissue lysate, human appendix tissue, human colon carcinoma tissue, human lung tissue, mouse lung tissue, rat lung tissue.

Molecular wt :

Predicted band size: 309 kDa

Subcellular location :

Secreted, extracellular space, extracellular matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:500 ;IF-Tissue: 1:100 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751166_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751166_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751166_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751166_7.jpg

Pic legend7 :

Immunofluorescence analysis of paraffin-embedded human appendix tissue labeling Von Willebrand Factor with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751166, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751166_8.jpg

Pic legend8 :

Immunofluorescence analysis of paraffin-embedded rat lung tissue labeling Von Willebrand Factor with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751166, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA751166_9.jpg

Pic legend9 :

Von Willebrand Factor was immunoprecipitated from 0.2 mg rat lung tissue lysate with HA751166 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751166 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: rat lung tissue lysate (input) Lane 2: HA751166 IP in rat lung tissue lysate Lane 3: Rabbit IgG instead of HA751166 in rat lung tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 20 seconds; ECL: K1801

more info or order :

HUABIO product webpage