SLUG | HUABIO HA751161 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SLUG

catalog :

HA751161

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-40

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SLUG on different lysates with Rabbit anti-SLUG antibody (HA751161) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: SK-MEL-28 cell lysate Lane 4: PANC-1 cell lysate (low expression) Lysates/proteins at 30 µg/Lane. Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 2 minutes 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751161) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of SK-MEL-28 (positive) and PANC-1 (low expression) labeling SLUG with Rabbit anti-SLUG antibody (HA751161) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLUG antibody (HA751161) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA751161

Target name :

SLUG

Species reactivity :

Human

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human SLUG aa 1-150.

Uniprot id :

O43623>SwissProt: O43623 Human

Host :

Rabbit

Clone number :

PSH07-40

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

U-87 MG cell lysate, SK-MEL-28 cell lysate, SK-MEL-28.

Molecular wt :

Predicted band size: 30 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage