product summary
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company name :
HUABIO
product type :
antibody
product name :
Retinoic Acid Receptor gamma
catalog :
HA751140
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH07-19
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/2,000 dilution.
Lane 1: SK-Br-3 cell lysate (20 µg/Lane)
Lane 2: T-47D cell lysate (20 µg/Lane)
Lane 3: A375 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: MEF cell lysate (20 µg/Lane)
Lane 6: Mouse skin tissue lysate (40 µg/Lane)
Lane 7: Rat skin tissue lysate (40 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 58 kDa
Exposure time: 1 minute 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751140) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/5,000 dilution.
Lane 1: Human RARG recombinant protein, 20ng/Lane
Lane 2: Human RARA recombinant protein, 20ng/Lane
Lane 3: Human RARB recombinant protein, 20ng/Lane
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751140) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751140) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA751140
Target name :
Retinoic Acid Receptor gamma
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human RARG aa 155-454.
Uniprot id :
P13631>SwissProt: P13631 Human;SwissProt: P18911 Mouse;Entrez Gene: 685072 Rat
Host :
Rabbit
Clone number :
PSH07-19
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SK-Br-3 cell lysate, T-47D cell lysate, A375 cell lysate, HeLa cell lysate, MEF cell lysate, Mouse skin tissue lysate, Rat skin tissue lysate, human skin tissue, mouse skin tissue, rat skin tissue.
Molecular wt :
Predicted band size: 50 kDa
Subcellular location :
Nucleus, Cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
;IHC-P: 1:1,000-1:5,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751140_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751140_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA751140) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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