HIF-1 alpha | HUABIO HA751127 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HIF-1 alpha

catalog :

HA751127

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-00

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (HA751127) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 100μM CoCl2 for 4 hours cell lysate Lane 5: C2C12 cell lysate Lane 6: C2C12 treated with 100μM CoCl2 for 4 hours cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751127) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with 0.5mM CoCl2 for 6 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (HA751127) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (HA751127) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of C2C12 cells treated with 200μM CoCl2 for 4 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (HA751127) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (HA751127) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA751127

Target name :

HIF-1 alpha

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human HIF-1 alpha aa 441-490.

Uniprot id :

Q16665>SwissProt: Q16665 Human;SwissProt: Q61221 Mouse

Host :

Rabbit

Clone number :

PSH07-00

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate, HepG2 cell lysate, HepG2 treated with 100μM CoCl2 for 4 hours cell lysate, C2C12 cell lysate, C2C12 treated with 100μM CoCl2 for 4 hours cell lysate, HeLa cells treated with 0.5mM CoCl2 for 6 hours, C2C12 cells treated with 200μM CoCl2 for 4 hours.

Molecular wt :

Predicted band size: 93 kDa

Subcellular location :

Cytoplasm, Nucleus, Nucleus speckle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751127_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded HeLa cells treated with 0.5mM CoCl2 for 6 hours with Rabbit anti-HIF-1 alpha antibody (HA751127) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751127) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage