product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
PKC zeta
catalog :
HA751122
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH06-74
reactivity :
human
application :
western blot
more info or order :
image
image 1 :

Western blot analysis of PKC zeta on different lysates with Rabbit anti-PKC zeta antibody (HA751122) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HL-60 cell lysate (negative)
Lane 3: HeLa cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 74 kDa
Exposure time: 1 minute 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751122) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA751122
Target name :
PKC zeta
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human PKC zeta aa 201-250.
Uniprot id :
Q05513>SwissProt: Q05513 Human
Host :
Rabbit
Clone number :
PSH06-74
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, A431 cell lysate.
Molecular wt :
Predicted band size: 68 kDa
Subcellular location :
Cytoplasm, Endosome, Cell junction, Membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
Advanced Validation :
Relative expression (RE)
more info or order :
company information

HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
related products
browse more products
questions and comments
