product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-p53 (S20)
catalog :
HA751110
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH06-86
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Phospho-p53 (S20) on different lysates with Rabbit anti-Phospho-p53 (S20) antibody (HA751110) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: HT-29 treated with UV then add 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA751110
Target name :
Phospho-p53 (S20)
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser20 of Human p53 aa 1-50 / 393.
Uniprot id :
P04637>SwissProt: P04637 Human
Host :
Rabbit
Clone number :
PSH06-86
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HT-29 treated with UV then add 100nM Calyculin A for 30 minutes cell lysate.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Cytoplasm, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix, cytoskeleton, microtubule organizing center, centrosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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