product summary
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company name :
HUABIO
product type :
antibody
product name :
ATP13A2
catalog :
HA751098
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH06-69
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
image
image 1 :
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
image 2 :
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
image 3 :
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
product information
SKU :
HA751098
Target name :
ATP13A2
Species reactivity :
Human,Mouse,Rat,Cynomolgus monkey
Applications :
WB,IHC-P,IHC-Fr
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ATP13A2 aa 485-900.
Uniprot id :
Q9NQ11>SwissProt: Q9NQ11 Human;SwissProt: Q9CTG6 Mouse;Entrez Gene: 362645 Rat
Host :
Rabbit
Clone number :
PSH06-69
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
mouse hippocampus tissue, mouse cerebral cortex tissue, HeLa cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, C6 cell lysate, COS-1 cell lysate, human brain tissue, mouse brain tissue, rat brain tissue.
Molecular wt :
Predicted band size: 129 kDa
Subcellular location :
Lysosome membrane, Late endosome membrane, Endosome, multivesicular body membrane, Cytoplasmic vesicle, autophagosome membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:1,000
;IHC-Fr: 1:500
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751098_4.jpg
Pic legend4 :
Western blot analysis of ATP13A2 on different lysates with Rabbit anti-ATP13A2 antibody (HA751098) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 129 kDa
Observed band size: 160 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751098) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751098_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ATP13A2 antibody (HA751098) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751098) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA751098_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ATP13A2 antibody (HA751098) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751098) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA751098_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ATP13A2 antibody (HA751098) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751098) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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