BRCC45/BRE | HUABIO HA751053 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BRCC45/BRE

catalog :

HA751053

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-15

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BRCC45/BRE on different lysates with Rabbit anti-BRCC45/BRE antibody (HA751053) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: Raji cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: HepG2 cell lysate (20 µg/Lane) Lane 7: K-562 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: Neuro-2a cell lysate (20 µg/Lane) Lane 10: C2C12 cell lysate (20 µg/Lane) Lane 11: PC-12 cell lysate (20 µg/Lane) Lane 12: C6 cell lysate (20 µg/Lane) Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 1 minute 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751053) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of PC-12 cells labeling BRCC45/BRE with Rabbit anti-BRCC45/BRE antibody (HA751053) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRCC45/BRE antibody (HA751053) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Flow cytometric analysis of HeLa cells labeling BRCC45/BRE. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751053, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA751053

Target name :

BRCC45/BRE

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human BRCC45 aa 1-383.

Uniprot id :

Q9NXR7>SwissProt: Q9NXR7 Human;SwissProt: Q8K3W0 Mouse;SwissProt: Q6P7Q1 Rat

Host :

Rabbit

Clone number :

PSH06-15

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, U-87 MG cell lysate, Raji cell lysate, 293T cell lysate, A431 cell lysate, HepG2 cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, PC-12, HeLa.

Molecular wt :

Predicted band size: 44 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50 ;FC: 1:1,000

more info or order :

HUABIO product webpage