ASGPR1 | HUABIO HA751041 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ASGPR1

catalog :

HA751041

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH06-01

reactivity :

human, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ASGPR1 on different lysates with Rabbit anti-ASGPR1 antibody (HA751041) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Human liver tissue lysate (40 µg/Lane) Lane 3: Rat liver tissue lysate (40 µg/Lane) Lane 4: Human brain tissue lysate (negative) (40 µg/Lane) Lane 5: Rat brain tissue lysate (negative) (40 µg/Lane) Predicted band size: 33 kDa Observed band size: 40-50 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751041) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling ASGPR1 with Rabbit anti-ASGPR1 antibody (HA751041) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ASGPR1 antibody (HA751041) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ASGPR1 antibody (HA751041) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751041) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751041

Target name :

ASGPR1

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human ASGPR1 aa 41-291.

Uniprot id :

P07306>SwissProt: P07306 Human;SwissProt: P02706 Rat

Host :

Rabbit

Clone number :

PSH06-01

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, Human liver tissue lysate, Rat liver tissue lysate, HepG2, human liver tissue, rat liver tissue.

Molecular wt :

Predicted band size: 33 kDa

Subcellular location :

Membrane; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:4,000 ;FC: 1:1,000 ;IF-Tissue: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751041_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-ASGPR1 antibody (HA751041) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751041) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751041_5.jpg

Pic legend5 :

ASGPR1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA751041 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751041 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA751041 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA751041 in HepG2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 20 seconds; ECL: K1801

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751041_6.jpg

Pic legend6 :

Flow cytometric analysis of HepG2 cells labeling ASGPR1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751041, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage