CD40 | HUABIO HA751037 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD40

catalog :

HA751037

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-90

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD40 on different lysates with Rabbit anti-CD40 antibody (HA751037) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: U-2 OS cell lysate Lane 4: HEK-293 cell lysate (negative) Lane 5: Ramos cell lysate Lane 6: A549 cell lysate (negative) Lane 7: Daudi cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 40 kDa Exposure time: Lane 1-2: 1 minute; Lane 3-7: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751037) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Raji cells labeling CD40 with Rabbit anti-CD40 antibody (HA751037) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD40 antibody (HA751037) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Flow cytometric analysis of Raji cells labeling CD40. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751037, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA751037

Target name :

CD40

Species reactivity :

Human,Cynomolgus monkey,Pig

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD40 aa 228-277 / 277.

Uniprot id :

P25942>SwissProt: P25942 Human

Host :

Rabbit

Clone number :

PSH05-90

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Raji cell lysate, U-2 OS cell lysate, Ramos cell lysate, Daudi cell lysate, Raji.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cell membrane. Membrane. Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage