product summary
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company name :
HUABIO
product type :
antibody
product name :
ANXA6
catalog :
HA751030
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH05-82
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of ANXA6 on different lysates with Rabbit anti-ANXA6 antibody (HA751030) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: Raji cell lysate (20 µg/Lane)
Lane 3: THP-1 cell lysate (20 µg/Lane)
Lane 4: PC-3M cell lysate (20 µg/Lane)
Lane 5: MCF7 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: HeLa cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: L6 cell lysate (20 µg/Lane)
Lane 10: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 11: Mouse liver tissue lysate (40 µg/Lane)
Lane 12: Rat heart tissue lysate (40 µg/Lane)
Lane 13: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 76 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-13 (left): 10 seconds; Lane 1-13 (right): 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751030) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
ANXA6 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA751030 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751030 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA751030 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA751030 in Jurkat cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 14 seconds; ECL: K1801
image 3 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ANXA6 antibody (HA751030) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751030) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA751030
Target name :
ANXA6
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human ANXA6 aa 1-673.
Uniprot id :
P08133>SwissProt: P08133 Human;SwissProt: P14824 Mouse;SwissProt: P48037 Rat
Host :
Rabbit
Clone number :
PSH05-82
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Jurkat cell lysate, Raji cell lysate, THP-1 cell lysate, PC-3M cell lysate, MCF7 cell lysate, HEK-293 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, L6 cell lysate, mouse pancreas tissue lysate, mouse liver tissue lysate, rat heart tissue lysate, rat liver tissue lysate, human liver tissue, human lung cancer tissue, human stomach tissue, mouse stomach tissue, rat stomach tissue.
Molecular wt :
Predicted band size: 76 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IHC-P: 1:2,000-1:10,000
;IF-Tissue: 1:500-1:2,000
;IP: 1-2μg/sample
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA751030_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ANXA6 antibody (HA751030) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751030) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA751030_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ANXA6 antibody (HA751030) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751030) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA751030_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-ANXA6 antibody (HA751030) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751030) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA751030_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-ANXA6 antibody (HA751030) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751030) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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