GIRK2 | HUABIO HA751027 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GIRK2

catalog :

HA751027

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-91

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

image 2 :

Western blot analysis of GIRK2 on different lysates with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate (no heat) Lane 4: Rat hippocampus tissue lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Predicted band size: 48 kDa Observed band size: 35/48 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751027) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

GIRK2 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA751027 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751027 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA751027 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA751027 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 26 seconds; ECL: K1801

product information

SKU :

HA751027

Target name :

GIRK2

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IP,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein.

Uniprot id :

P48051>SwissProt: P48051 Human;SwissProt: P48542 Mouse;SwissProt: P48550 Rat

Host :

Rabbit

Clone number :

PSH05-91

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse brain tissue lysate, mouse hippocampus tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IP: 1-2μg/sample ;IHC-Fr: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751027_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751027_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751027_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751027_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751027_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-GIRK2 antibody (HA751027) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage