LAMP1 | HUABIO HA751017 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

LAMP1

catalog :

HA751017

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-55

reactivity :

mouse

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA751017) at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 30 µg/Lane. Predicted band size: 44 kDa Observed band size: 100-120 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751017) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IHC-Fr Species: Mouse Site: Dorsal root ganglion Sample: Frozen section Antibody concentration: 1: 500 Date by conrtesy of: Mr. Rongyao Ye, School of Basic Medical Sicences, Zhejiang University

image 3 :

Application: IHC-Fr Species: Mouse Site: Colon Sample: Frozen section Antibody concentration: 1: 500 Antigen retrieval: Not required

product information

SKU :

HA751017

Target name :

LAMP1

Species reactivity :

Mouse

Applications :

WB,IF-Cell,IHC-P,FC,IP,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein.

Uniprot id :

P11438>SwissProt: P11438 Mouse

Host :

Rabbit

Clone number :

PSH05-55

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

C2C12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, NIH/3T3, mouse colon tissue, mouse kidney tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 44 kDa

Subcellular location :

Lysosome membrane, Endosome membrane, Late endosome membrane, Cell membrane, Cytolytic granule membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000 ;FC: 1:1,000 ;IP: 1-2μg/sample ;IF-Tissue: 1:200 ;IHC-Fr: 1:500-1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA751017_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling LAMP1 with Rabbit anti-LAMP1 antibody (HA751017) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LAMP1 antibody (HA751017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA751017_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LAMP1 antibody (HA751017) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751017) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA751017_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-LAMP1 antibody (HA751017) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751017) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA751017_7.jpg

Pic legend7 :

Flow cytometric analysis of NIH/3T3 cells labeling LAMP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751017, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA751017_8.jpg

Pic legend8 :

LAMP1 was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA751017 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751017 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: HA751017 IP in NIH/3T3 cell lysate Lane 3: Rabbit IgG instead of HA751017 in NIH/3T3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801

more info or order :

HUABIO product webpage