product summary
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company name :
HUABIO
product type :
antibody
product name :
GPR56
catalog :
HA751002
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH05-43
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA751002 image 1
Western blot analysis of GPR56 on different lysates with Rabbit anti-GPR56 antibody (HA751002) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: A-172 cell lysate Lane 2: U-87 MG cell lysate Lane 3: T-47D cell lysate Lane 4: Human liver tissue lysate (negative) Lane 5: Human brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 78 kDa Observed band size: 55-70 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751002) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA751002 image 2
Immunohistochemical analysis of paraffin-embedded human glioma tissue with Rabbit anti-GPR56 antibody (HA751002) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751002) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA751002
Target name :
GPR56
Species reactivity :
Human
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human GPR56 aa 26-402.
Uniprot id :
Q9Y653>SwissProt: Q9Y653 Human
Host :
Rabbit
Clone number :
PSH05-43
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A-172 cell lysate, U-87 MG cell lysate, T-47D cell lysate, human brain tissue lysate, human glioma tissue.
Molecular wt :
Predicted band size: 78 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;IHC-P: 1:1,000
Advanced Validation :
Relative expression (RE)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!