Phospho-Tau (T181) | HUABIO HA751001 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-Tau (T181)

catalog :

HA751001

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-42

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-Tau (T181) on different lysates with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 40 µg/Lane. Predicted band size: 79 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751001) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751001) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751001) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA751001

Target name :

Phospho-Tau (T181)

Species reactivity :

Human,Mouse,Cynomolgus monkey

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding T181 of human Tau protein(P10636-8).

Uniprot id :

P10636-8>SwissProt: P10636-8 Human;SwissProt: P10637 Mouse

Host :

Rabbit

Clone number :

PSH05-42

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue lysate, human brain tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 79 kDa

Subcellular location :

Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:50

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage