KDM2B | HUABIO HA750997 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

KDM2B

catalog :

HA750997

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-38

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of KDM2B on different lysates with Rabbit anti-KDM2B antibody (HA750997) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C2C12 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: Human brain tissue lysate (40 µg/Lane) Lane 9: Mouse brain tissue lysate (40 µg/Lane) Lane 10: Mouse colon tissue lysate (40 µg/Lane) Lane 11: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 150 kDa Observed band size: 150 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750997) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling KDM2B with Rabbit anti-KDM2B antibody (HA750997) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KDM2B antibody (HA750997) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells labeling KDM2B with Rabbit anti-KDM2B antibody (HA750997) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KDM2B antibody (HA750997) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750997

Target name :

KDM2B

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IF-Tissue,IP,ChIP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse KDM2B aa 681-920 / 1,309.

Uniprot id :

Q8NHM5>SwissProt: Q8NHM5 Human;SwissProt: Q6P1G2 Mouse;Entrez Gene: 304495 Rat

Host :

Rabbit

Clone number :

PSH05-38

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HepG2 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, mouse colon tissue lysate, rat brain tissue lysate, HeLa, NIH/3T3, human brain tissue, human colon tissue, mouse brain tissue, mouse skin tissue, rat brain tissue.

Molecular wt :

Predicted band size: 150 kDa

Subcellular location :

Nucleus, nucleolus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:2,000 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IF-Tissue: 1:50-1:200 ;IP: 1-2μg/sample ;ChIP: Use 5 μg for 25 μg of chromatin.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750997_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-KDM2B antibody (HA750997) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750997) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750997_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KDM2B antibody (HA750997) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750997) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750997_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-KDM2B antibody (HA750997) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750997) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750997_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-KDM2B antibody (HA750997) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750997) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750997_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-KDM2B antibody (HA750997) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750997) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750997_9.jpg

Pic legend9 :

Flow cytometric analysis of HeLa cells labeling KDM2B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750997, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750997_10.jpg

Pic legend10 :

Flow cytometric analysis of NIH/3T3 cells labeling KDM2B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750997, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750997_11.jpg

Pic legend11 :

KDM2B was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with HA750997 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750997 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MDA-MB-231 cell lysate (input) Lane 2: HA750997 IP in MDA-MB-231 cell lysate Lane 3: Rabbit IgG instead of HA750997 in MDA-MB-231 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1802

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA750997_12.jpg

Pic legend12 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from MDA-MB-231 cells with KDM2B (HA750997) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage