product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
ATG4C
catalog :
HA750994
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH05-23
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of ATG4C on different lysates with Rabbit anti-ATG4C antibody (HA750994) at 1/2,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: HeLa cell lysate (20 µg/Lane)
Lane 6: K-562 cell lysate (20 µg/Lane)
Lane 7: HL-60 cell lysate (20 µg/Lane)
Lane 8: Mouse brain tissue lysate (40 µg/Lane)
Lane 9: Rat brain tissue lysate (40 µg/Lane)
Lane 10: COS-1 cell lysate (20 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750994) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
ATG4C was immunoprecipitated from 0.2 mg Raji cell lysate with HA750994 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750994 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate (input)
Lane 2: HA750994 IP in Raji cell lysate
Lane 3: Rabbit IgG instead of HA750994 in Raji cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 21 seconds; ECL: K1801
product information
SKU :
HA750994
Target name :
ATG4C
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ATG4C aa 1-458 / 458.
Uniprot id :
Q96DT6>SwissProt: Q96DT6 Human;SwissProt: Q811C2 Mouse;Entrez Gene: 313391 Rat
Host :
Rabbit
Clone number :
PSH05-23
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T cell lysate, Jurkat cell lysate, Raji cell lysate, A431 cell lysate, HeLa cell lysate, K-562 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, COS-1 cell lysate.
Molecular wt :
Predicted band size: 52 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments