Fast Myosin Skeletal Heavy chain | HUABIO HA750985 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Fast Myosin Skeletal Heavy chain

catalog :

HA750985

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-12

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue / human brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA750985) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750985) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue / mouse brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA750985) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750985) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue / rat brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA750985) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750985) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750985

Target name :

Fast Myosin Skeletal Heavy chain

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Fast Myosin Skeletal Heavy chain aa

Uniprot id :

P12882>SwissProt: P12882 Human;SwissProt: Q5SX40 Mouse;Entrez Gene: 287408 Rat

Host :

Rabbit

Clone number :

PSH05-12

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.

Molecular wt :

Predicted band size: 223 kDa

Subcellular location :

Cytoplasm, myofibril.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:2,000-1:10,000 ;IF-Tissue: 1:400-1:2,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage