TPH2 | HUABIO HA750976 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TPH2

catalog :

HA750976

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH04-89

reactivity :

mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Raphe nucleus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse midbrain tissue with Rabbit anti-TPH2 antibody (HA750976) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750976) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-TPH2 antibody (HA750976) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750976) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750976

Target name :

TPH2

Species reactivity :

Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein with Human THP2 aa 1-490.

Uniprot id :

Q8CGV2>SwissProt: Q8CGV2 Mouse;SwissProt: Q8CGU9 Rat

Host :

Rabbit

Clone number :

PSH04-89

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse hippocampus tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse midbrain tissue, mouse cerebrum tissue, rat cerebrum tissue, mouse brain (raphe nucleus) tissue.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Cytosol, neuron projection.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:500 ;IHC-Fr: 1:500 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750976_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-TPH2 antibody (HA750976) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750976) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750976_5.jpg

Pic legend5 :

Western blot analysis of TPH2 on different lysates with Rabbit anti-TPH2 antibody (HA750976) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate (negative) Lane 3: Rat brain tissue lysate Lane 4: Rat kidney tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750976) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750976_6.jpg

Pic legend6 :

TPH2 was immunoprecipitated from 0.2 mg rat brain tissue lysate with HA750976 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750976 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: rat brain tissue lysate (input) Lane 2: HA750976 IP in rat brain tissue lysate Lane 3: Rabbit IgG instead of HA750976 in rat brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801

more info or order :

HUABIO product webpage