Lrp2 / Megalin | HUABIO HA750959 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Lrp2 / Megalin

catalog :

HA750959

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH04-52

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Lrp2 / Megalin on different lysates with Rabbit anti-Lrp2 / Megalin antibody (HA750959) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750959) at 1/2,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Lrp2 / Megalin antibody (HA750959) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Lrp2 / Megalin antibody (HA750959) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750959

Target name :

Lrp2 / Megalin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Lrp2 aa 2,500-3,200 / 4,655.

Uniprot id :

P98164>SwissProt: P98164 Human;SwissProt: A2ARV4 Mouse;SwissProt: P98158 Rat

Host :

Rabbit

Clone number :

PSH04-52

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human kidney tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 522 kDa

Subcellular location :

Apical cell membrane, Endosome lumen, Membrane, coated pit, Cell projection, dendrite, axon.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750959_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Lrp2 / Megalin antibody (HA750959) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750959_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Lrp2 / Megalin antibody (HA750959) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750959_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (negative) with Rabbit anti-Lrp2 / Megalin antibody (HA750959) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750959_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue (negative) with Rabbit anti-Lrp2 / Megalin antibody (HA750959) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage