Phospho-STAT1 (Y701) | HUABIO HA750925 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-STAT1 (Y701)

catalog :

HA750925

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH04-02

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-STAT1 (Y701) on different lysates with Rabbit anti-Phospho-STAT1 (Y701) antibody (HA750925) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL IFNγ for 30 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 50ng/mL IFNα1 for 30 minutes cell lysate Lane 5: A431 cell lysate Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 91/84 kDa (STAT1α/β) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750925) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with 100ng/mL IFNα1 for 5 minutes labeling Phospho-STAT1 (Y701) with Rabbit anti-Phospho-STAT1 (Y701) antibody (HA750925) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-STAT1 (Y701) antibody (HA750925) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750925

Target name :

Phospho-STAT1 (Y701)

Species reactivity :

Human

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Tyr701 of Human STAT1.

Uniprot id :

P42224>SwissProt: P42224 Human

Host :

Rabbit

Clone number :

PSH04-02

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa treated with 100ng/mL IFNγ for 30 minutes cell lysate, HeLa treated with 50ng/mL IFNα1 for 30 minutes cell lysate, A431 treated with 100ng/mL EGF for 30 minutes cell lysate, HeLa cells treated with 100ng/mL IFNα1 for 5 minutes.

Molecular wt :

Predicted band size: 87 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage