CPT1A | HUABIO HA750923 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CPT1A

catalog :

HA750923

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH04-00

reactivity :

human, mouse

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CPT1A on different lysates with Rabbit anti-CPT1A antibody (HA750923) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: SK-OV-3 cell lysate Lane 4: MCF7 cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: Human kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750923) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CPT1A antibody (HA750923) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750923) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-CPT1A antibody (HA750923) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750923) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750923

Target name :

CPT1A

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CPT1A aa 201-773 / 773.

Uniprot id :

P50416>SwissProt: P50416 Human;SwissProt: P97742 Mouse

Host :

Rabbit

Clone number :

PSH04-00

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, HeLa cell lysate, SK-OV-3 cell lysate, MCF7 cell lysate, HepG2 cell lysate, A549 cell lysate, Human kidney tissue lysate, human kidney tissue, human ovary cancer tissue, mouse brain tissue, mouse hippocampus tissue, mouse kidney tissue.

Molecular wt :

Predicted band size: 88 kDa

Subcellular location :

Mitochondrion outer membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:500-1:2,000 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750923_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CPT1A antibody (HA750923) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750923) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750923_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CPT1A antibody (HA750923) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750923) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750923_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CPT1A antibody (HA750923) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750923) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750923_7.jpg

Pic legend7 :

CPT1A was immunoprecipitated from 0.2 mg HeLa cell lysate with HA750923 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750923 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA750923 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA750923 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 seconds; ECL: K1801

more info or order :

HUABIO product webpage